|SR NO||TEST||TEST COMPONENTS||METHOD||SPECIMEN/TRANSPORT||TAT||CLINICAL APPLICATIONS|
|1||AFB||AFB||Conventional microscopic examination after ZN stain||All specimens except blood||Report Same Day||Detection of Mycobacterium spp (AFB) by Microscopy|
|2||AFB CULTURE||AFB stain from concentration material. Differentiation of Mycobacterium between MTB and NTM/MOTT||Liquid rapid culture on MGIT. Followed by Differentiation between MTB and NTM/MOTT by TBMPT64Ag Card test.||All specimens except blood/Pus/Body fluids (Pleural/Pericardial/Ascitic/ Synovial/Ocular) Aspirates /Semen/ BAL Bronchial washings: Submit as much as possible (1 mL min.) in sterile screw capped container. Ship refrigerated.|
Tissue: Submit in sterile normal saline in a sterile screw capped container. Ship refrigerated.
Swabs: Submit swabs in 1 mL sterile normal saline in sterile screw capped container. Ship refrigerated.
|Priliminary reports at 10 and 20 days and final repot at 42 days or earlier if positive. If smear positive for AFB and culture negative till 42 days then final report at 56 days.||Detection of Mycobacterium spp. (MTB or NTM/MOTT) from any clinical specimen|
|3||AFBIF||AFB BY FLUORSCENT STAIN||Fluorescent stain and Fluorescent microscopy||Miscellaneous specimens ( Urine, Pus, Sputum, Body Fluids like pleural, peritoneal, pericardial, gastric, bronchial, semen etc.) in a sterile, screw capped container OR Tissue in sterile normal saline OR Swabs in sterile normal saline||Report Same Day||Detection of AFB.
Immunoflourescense increases detection chances by Microscopy (Increased sensitivity of detection as compared to light microscopy)
|4||ANAEROBIC CULTURE||Gram stain, ZN stain, KOH, Anaerobic growth if any. Identification of anaerobic organism on MALDI TOF||Conventional anaerobic culture and automatic identification on MALDI TOF after growth||All sample except blood (Blood anaerobic culture is done by BACTEC method under entry Batctec anaerobic)|
Collection and transportation under strict anerobic condition. Dry swab not allowed. Anaerobic transport medium is required.
|5 days||Isolation of Anaerobic organism|
|5||BACTEC AEROBIC||-||Rapid Automated Aerobic Culture on BACTEC FX 200, Identification and Sensitivity||BLOOD/8-10ML/CULTURE BOTTLE. or FLUID/PUS/TISSUE in CULTURE BOTTLE/STERILE CONTAINER|
Collect 8-10 mL blood aseptically in special Plus Aerobic bottle available from SMPL. Mix by gentle swirling. Ship at room temperature.
|Aerobic culture which includes enrichment, which increases detection chances as compared to platting method in case of fluid/pus/tissue. Also includes aerobic culture of blood. Long incubation can be done in case of request of recovery of small organisms|
|6||BACTEC ANAEROBIC||-||Rapid Automated Anaerobic Culture on BACTEC FX 200 and Identification||BLOOD/8-10ML/CULTURE BOTTLE. or FLUID/PUS/TISSUE in CULTURE BOTTLE/STERILE CONTAINER|
Collect 8-10 mL blood aseptically in special Plus Anerobic bottle. Mix by gentle swirling. Ship at room temperature.
|Anaerobic culture which includes enrichment, which increases detection chances as compared to platting method in case of fluid/pus/tissue. Also includes anerobic culture of blood|
|7||BACTEC MYCO||-||Rapid Automated fungal Culture/NTM culture on BACTEC FX 200||BLOOD/1-5ML/CULTURE BOTTLE. or FLUID/PUS/TISSUE in CULTURE BOTTLE/STERILE CONTAINER|
Collect 8-10 mL whole blood / body fluids aseptically in a Myco / F-Lytic bottle available from ADL. Mix by gentle swirling. Ship at room temperature.
|Rapid culture system for detection of yeast/fungus/MOTT/NTM from blood and other body fluid.|
|8||Calcoflour Staininig||CALCOFLUOR STAININIG||Fluorescent microscopy||All specimens except Blood||6 hrs||Highly sensitive method for detection of Yeast or Fungus by Florescent staining.|
|9||FUNGUS CULTURE||Gram stain, AFB stain, KOH, Identification of fungus grown, Colony count||Conventional Fungus Culture and Rapid Culture for Yeast.||CSF: 2 mL (1 mL min.) in a Red Top (No Additive) tube or sterile screw capped vial. Do not use SST gel barrier tubes. Ship refrigerated. Bone Marrow: 2 mL (0. 5 mL min.) Bone marrow in 1 Green Top (Sodium Heparin) tube. Ship refrigerated. Pus/Body fluids (Pleural/ Pericardial/ Ascitic/ Synovial/ Ocular/ Bronchial washings/BAL). Aspirates /Semen/ Stool: Submit as much as possible (1 mL/1g min.) in sterile screw capped container. Ship refrigerated. Endometrial curettings/Tissue: Submit in sterile normal saline in a sterile screw capped container. Ship refrigerated. Swabs: Submit swabs in 1 mL sterile normal saline in sterile screw capped container. Ship refrigerated. Sputum/Urine: Submit 2 Spot (random) morning samples, 5–10 mL (1 mL min.) sputum/10 mL (5 mL min.) urine in sterile screw capped container. Ship refrigerated. Skin: Submit 2–3 mm scrapings from periphery of active skin lesion in sterile container or between 2 sterile glass slides. Wrap slides in black paper. Ship refrigerated. Hair: Submit plucked 10–12 infected, lustreless hair including root in a sterile container. Ship refrigerated. Nail: Submit clippings and powdery material from friable and discolored region of affected nail. Ship refrigerated.||10 days||Detection of fungal infection by culture method.|
|10||Genexpert-Tb||MTB detection, Rifampicin susceptibility||RT-PCR by GeneXpert||Any specimen except blood/2ml/Sterile container|
In case of Bone marrow, sample is to be collected in sterile container containing distilled water in double volume of bone marrow sample
|6 hrs. Next day after cut off time||Highly sensitive and specific method for rapid detection of MTB infection and Rif susceptibility, directly from clinical specimen.|
|11||Gram - Stain||GRAM STAIN EXAMINATION :||Light Microscopy after gram stain||Any specimen except blood/1ml/Sterile container||4 hrs||Detection of any microorganism and pus cells by microscopic examination.|
|12||KOH PREPARATION||Light Microscopy||Submit Body fluid, Sputum, Bronchial washings / Tissue in a sterile screw capped container. Ship refrigerated.||4 hrs||Detection of Fungal infection in clinical specimen by Microscopy.|
|13||LPA (Line Probe Assay) MTB-MDR||LPAMTBMDR||PCR followed by Membrane hybridization||Any AFB smear positive sample||4 days||Detection of MTB infection by PCR + Hybridization method along with detection of Isonizide and Rifampicin drug susceptibility status directly from specimen.|
|14||MODIFIED ZN STAIN||-||Light Microscopy||Sputum, Pus, Tissue, BAL for Nocardia. Slit skin smears for Lepra bacilli.||4 hrs||For detection of Nocardia spp in clinical specimen.|
|15||PRIMARY STAIN||Gram stain, AFB stain, KOH, AFB by IF, Calcofluor white||Microscopic examination after various stains||Any specimen except blood||4 hrs||All primary stains for detection of any infectious agent from clinical specimen.|