Microbiology

Microbiology

SR NOTESTTEST COMPONENTSMETHODSPECIMEN/TRANSPORTTATCLINICAL APPLICATIONS
1Calcoflour StaininigCALCOFLUOR STAININIGFluorescent microscopyAll specimens except Blood6 hrsHighly sensitive method for detection of Yeast or Fungus by Florescent staining.
2Chlamydia and N.Gonorrohoeae By Gene expertChlamydia trachomatis, Neisseria gonorrhoeaeRT-PCR by GeneXpertUrine in sterile container, Vaginal swab/ Endocervical swab in transport medium 20-30 ml of Urine or Vaginal or endocervical swab in transport kit4 hrsMolecular method for detection of N. Gonorrohoeae and C. trachomatis
3FUNGUS CULTUREGram stain, AFB stain, KOH, Identification of fungus grown, Colony countConventional Fungus Culture and Rapid Culture for Yeast.CSF: 2 mL (1 mL min.) in a Red Top (No Additive) tube or sterile screw capped vial. Do not use SST gel barrier tubes. Ship refrigerated. Bone Marrow: 2 mL (0. 5 mL min.) Bone marrow in 1 Green Top (Sodium Heparin) tube. Ship refrigerated. Pus/Body fluids (Pleural/ Pericardial/ Ascitic/ Synovial/ Ocular/ Bronchial washings/BAL). Aspirates /Semen/ Stool: Submit as much as possible (1 mL/1g min.) in sterile screw capped container. Ship refrigerated. Endometrial curettings/Tissue: Submit in sterile normal saline in a sterile screw capped container. Ship refrigerated. Swabs: Submit swabs in 1 mL sterile normal saline in sterile screw capped container. Ship refrigerated. Sputum/Urine: Submit 2 Spot (random) morning samples, 5–10 mL (1 mL min.) sputum/10 mL (5 mL min.) urine in sterile screw capped container. Ship refrigerated. Skin: Submit 2–3 mm scrapings from periphery of active skin lesion in sterile container or between 2 sterile glass slides. Wrap slides in black paper. Ship refrigerated. Hair: Submit plucked 10–12 infected, lustreless hair including root in a sterile container. Ship refrigerated. Nail: Submit clippings and powdery material from friable and discolored region of affected nail. Ship refrigerated.10 daysDetection of fungal infection by culture method.
4FUNGUS MICAntifungal susceptibility with MIC value and interpretation of Posaconazole, Amphotericin-B, Fluconazole, Itraconazole, Ketoconazole, Flucytosine, Voriconazole, CaspofunginBroth microdilutionPure growth of Yeast from any sample72 hrsSusceptibility of Antifungal drugs against any Yeast or Aspergillus spp, by MIC method
5Gram - StainGRAM STAIN EXAMINATION :Light Microscopy after gram stainAny specimen except blood/1ml/Sterile container4 hrsDetection of any microorganism and pus cells by microscopic examination. Gram stain from Vaginal swab for diagnosis of Bacterial vaginosis (Nugent score).
6IDENTIFICATION BY MALDI TOF (Mass Spectrometry)Identification of organism by MALDI TOFMass spectrometryUpto 48 hrs of culture growth or any bacteria or yeast. Growth on Blood agar or LJ medium or MGIT in case of NTM or MTB. Hyphal growth on plate or slant.2 hrsUltra rapid identification of any Bacteria, Yeast, Hyphal fungus, Mycobacteium spp from growth
7KOH PREPARATIONLight MicroscopySubmit Skin, Nail scrapings, Hair ( including root), Cerebrospinal fluid, in a sterile screw capped container OR 2 mL whole blood / bone marrow in 1 Green Top (Sodium Heparin) tube. Ship at 18–22°C. Submit Body fluid, Sputum, Bronchial washings / Tissue in a sterile screw capped container OR Oral/Genital/wound swabs in 0. 5 mL sterile normal saline. Ship refrigerated. 4 hrsDetection of Fungal infection in clinical specimen by Microscopy.
8PYOGENIC CULTURE AND SENSITIVITY FOR BACTERIAGram stain, ZN stain, Oragnism isolated, Colony countAerobic culture and susceptibility repoting of bacteria by Kirby bauer methodAny specimen except blood48-72 hrsCulture of bacteria from specimen along with its identification, colony count and susceptibility by Kirby bauer method.
9AUTOI (Identification on Vitek)-Automated identification on Vitek 2 compact systemPure growth of bacteria or yeast on plate or slant Submit pure isolate on appropriate media. Ship refrigerated. DO NOT FREEZE. Specify collection site and provide brief clinical history.48 hrsIdentification of Bacteria/Yeast by automated system
10SPECIMEN FOR NEISSERIA GONORRHOEAE BY MICROSCOPYGram stain, Light microscopyUrethral discharge/ Urine2 hrsDetection of N. gonorrheae from urine or urethral discharge by gram staining
11Chlamydia antigen-Immunochromatographic assayEndocervical swab / Cytology brush4 hrsDetection of Chlamydia antigen from Endocervical swab or Cytology brush