Histopathology

Histopathology

SR NOTESTTEST COMPONENTSMETHODSPECIMEN/TRANSPORTTATCLINICAL APPLICATIONS
1Frozen section-On Cryostate Prior notification of frozen section required for
1)     Time of frozen section
2)     Type of specimen will be sent to us for frozen
3)     What surgeon wants from frozen section
4)     Relevant clinical details
5)     Contact no of surgeon for communication during and after frozen
Fresh specimen in a container without formalin35 mins after it reaches our laboratoryDiagnosis and decision making for additional surgery requirement
2Immunohistochemistry studyA)    Mesothelioma V/S Metastatic adenocarcinoma
·  Calretinin
·  CK5/6
·  CEA
·  Ber2EP4
·  CD15
·  PAN CK
·  CK7
·  CK20
B)     GIT
· CK7
· CK20
· Pan CK
· CK19
· CDX2
· Hepar
· AFP
· Glypican 3
· Her2Neu
· CD117
· DOG1
· H Caldesmon
· CEA
· CD34
C) MMR IHC
Leica BondMax fully automated IHC machineFormalin fixed tissue3-4 daysFor definitive diagnosis suspected on routine histopathology stain.
3Liquid based cytology and cell block preparation (All body fluid)-By special technique background material is reduced and hence individual cells can be seen clearly to detect early cancerSpecimen to be collected in plain tube and transported as early as possible. In case of delay keep in refrigerator and transport with coolent.24 hrsHelps early detection of cancer by better visualization of individual cells
4Special stainsPAS stain-for fungal elements
ZN stain for acid fast bacilli
ManualFormalin fixed tissue48 to 72 hrs, may be longer if longer bone decalcification requiredFor detection of infective pathology
5Same day biopsy reportingSmall biopsyMicrowave based testFormalin fixed tissue to be received before 12.00 noon and report will be done by same day before 7 o’clock
6FNACa)     Guided only Smears reporting FNAC procedurea)     Received from radiologist or surgeons
b)     OPD procedure at our centre
a)     With proper fixatives
b)     Smears prepared from superficially palpable lesions