Histopathology

SR NOTESTTEST COMPONENTSMETHODSPECIMEN/TRANSPORTTATCLINICAL APPLICATIONS
1Frozen sectionEndometrial, cervical, ovarian mass (with limitations of requirement of permanent sections as the case)On Cryostate
Prior notification of frozen section required for
1) Time of frozen section
2) Type of specimen will be sent to us for frozen
3) What surgeon wants from frozen section
4) Relevant clinical details
5) Contact no of surgeon for communication during and after frozen
Fresh specimen in a container without formalin35 mins after it reaches our laboratoryDiagnosis and decision making for additional surgery requirement
2Liquid based cytology and cell block preparation ( Gynec and non gynec -All body fluid)-By special technique background material is reduced and hence individual cells can be seen clearly to detect early cancerSpecimen to be collected in plain tube and transported as early as possible. In case of delay keep and refrigerator and transport with coolent.24 hrsHelps early detection of cancer by better visualization of individual cells
3Immunohistochemistry studyA)    Germ Cell tumor
· AFP
· PLAP
· CD117
· CD30
B) Ovarian/gynec tumor
· CA125
· Inhibin
· WT1
· Calretinin
· P53
· P16
· CEA
· CD56
· CK7
· CK20
· CK 5/6
· ER
· PR
· Pan CK
Leica Bond Max fully automated IHC machineFormalin fixed tissue with tumor or paraffin blocks of tumor tissue
Cell block of body fluids
3-5 daysHelps in diagnostic as well as therapeutic purpose
4Special stainZN stain for acid fast bacilli PAS stain: for fungal infection
ManualFormalin fixed tissue or paraffin block24 to 48 hrsFor infective pathology.