Histopathology

Histopathology

SR NOTESTTEST COMPONENTSMETHODSPECIMEN/TRANSPORTTATCLINICAL APPLICATIONS
1Special stainsPAS stain
PASM stain
MT stain
Congo-red stain
Manual
Sections cut on microtome at various thickness as required for special stain
Formalin fixed tissue/paraffin blocks of tissue24 hrsFor definite diagnosis of clinically suspected on routine H &E stain.
2Immunofluorescence studyIgG, IgA, IgM, C3, C1q, Kappa, Lambda, Fibrinogen
Albumin
Sections cut on cryostatePhosphate buffer saline or Michell media
Trnasport with coolent
24 hrsFor further typing of glomerular disease diagnosed on routine H &E stain
3Immunohistochemistry study.IgA, SAA
IgG4
Kappa
Lambda
C4d
Sections cut on microtome at various thicknessFormalin fixed tissue/paraffin blocks of tissue24 to 48 hrsFor definitive diagnosis of IgA nephropathy if IF can not be done with IgA, for IgG4 disease and for differentiating porimary from secondary amyloidosis.
4Electron MicroscopyOutsourcedOusourced to Jaslok hospitalGluteraldehyde with coolent10-12 daysFor detection of diseases liked thin basement membrane disease, Fibrillary disease, DDD etc