Histopathology

Histopathology

SR NOTESTTEST COMPONENTSMETHODSPECIMEN/TRANSPORTTATCLINICAL APPLICATIONS
1Liquid based cytology (body fluids-pleural fluid, ICD drain )-cytocentrifugeIn and plain container, transport immediately if delay anticipated store in refrigerator and transport with coolent24 To 48 hrsFor better detection of early cancer or infective organisms
2Cell block preparation from body fluids-By LBC techniqueSame as above24 to 48 hrsFor doing immunohistochemistry and genetic tests from cell block as and when required
3·       Immunohistochemistry studyA)       Lung tumor Panel
· TTF1,
·  Napsin
·  CK7
·  Ck20
·  CK HMW
·  CK5/6
·  Calretinine
·  P63
B) Mesothelioma V/S Metastatic adenocarcinoma
·   Calretinin
·  CK5/6
·  CEA
·  Ber2EP4
·  CD15
·  PAN CK
·  CK7
·  CK20
C) Infective pathology
·  CMV
·  EBV
Leica Bond max Fully automated IHC MachineSame as above3-4 daysFor confirmation of diagnosis for targeted therapy
4Special stainsPAS for fungal elements
ZN stain for acid fast bacilli
Retic stain for pneumocystis carini
Iron stain for hosiderosis
Manual methodSame as above24 hrsHelps clinician decide on infective pathology treatment